Coding

Part:BBa_M11033:Design

Designed by: Erik Ostler   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-17)

Endo-1,4-beta-glucanase B [Clostridium thermocellum]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 254
    Illegal AgeI site found at 701
    Illegal AgeI site found at 1304
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was imported into DNA 2.0 and the best nucleotides for E. coli were determined. An ribosome binding site, secretion signal, and two stop codons were added to the part


Source

Amino acid sequence was obtained from the NCBI database.

References

http://www.ncbi.nlm.nih.gov/protein/121815